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Biotechnology Information ncbi nucleotide collection nr nt database
Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( <t>https://www.ncbi.nlm.nih.gov/nuccore/KX011115</t> ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end <t>nucleotide</t> positions within the KX01115 sequence.
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Article Title: On the sensitivity of qPCR diagnostics for the canola clubroot pathogen Plasmodiophora brassicae

Journal: bioRxiv

doi: 10.64898/2026.02.05.704046

Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.
Figure Legend Snippet: Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.

Techniques Used: Sequencing, Synthesized

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Synthesized:

Article Title: Metagenomics Reveals That Intravenous Injection of Beta-Hydroxybutyric Acid (BHBA) Disturbs the Nasopharynx Microflora and Increases the Risk of Respiratory Diseases
Article Snippet: To analyze the species composition, the scaffolds/scaftigs of each sample were subjected to BLASTN (“E < 0.00001”) against the bacterial, archaeal, fungal, and virus sequences in the NCBI-NT database (National Centre for Biotechnology Information–Nucleotide Collection, v2016-6-19).

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Article Title: Ophiostoma haidaense , sp. nov., a new member of the O. piceae species complex associated with yellow-cedar, Callitropsis nootkatensis .
Article Snippet: Ophiostoma haidanensis is described as a new species of the Ophiostoma piceae complex isolated from yellow-cedar (Callitropsis nootkatensis (D. Don) Oerst. ex D.P.. Little) sapwood in the Haida Gwaii island archipelago and the North Coast of British Columbia, Canada.. The fungus is characterized by the production of a typical sporothrix-like asexual morph but is distinguished morphologically from other members of the O. piceae species complex by its large, multiseptate primary conidia.



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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.

Journal: bioRxiv

Article Title: On the sensitivity of qPCR diagnostics for the canola clubroot pathogen Plasmodiophora brassicae

doi: 10.64898/2026.02.05.704046

Figure Lengend Snippet: Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.

Article Snippet: The target regions of the primers were obtained by BLAST searching the primer sequences against the National Center for Biotechnology Information (NCBI) nucleotide collection (nr/nt) database ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Techniques: Sequencing, Synthesized